Determination of Transdermal Osmolality of Metoprolol Tartrate by Second-order Derivative UV Spectrophotometry in Vitro
- VernacularTitle:二阶导数紫外分光光度法测定酒石酸美托洛尔体外经皮渗透量
- Author:
Wengang WANG
;
Liuhong YUN
- Publication Type:Journal Article
- Keywords:
Second-order derivative UV-spectrophotometry;
Metoprolol tartrate;
Transdermal permeation
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the Second-order Derivative UV-spectrophotometry for the determination of transdermal osmolality of metoprolol tartrate (MP)in vitro.METHODS:The quantitation of the vibration amplitude (?A)between the characteristic valley and the characteristic peak of MP appeared at 224nm and 234nm in UV-scanning.RESULTS:The linear concentration range of metoprolol tartrate was 5～60?g/ml,the average recovery was 98.89%with average RSD at 2.05%.CONCLUSION:This method can accurately determine the transdermal osmolality of MP in vitro yet without the interference of skin exudates,its operation is simple and fast and the results are accurate and reliable.
