Determination of Betahistine in Human Plasma by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定人血浆中倍他司汀浓度
- Author:
Chun PAN
- Publication Type:Journal Article
- Keywords:
Betahistine;
RP-HPLC;
Blood concentration
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish RP-HPLC method for the determination of betahistine in human plasma.METHODS:The separation of betahistine was performed on C18 column with a detection wavelength of 261nm.the mobile phase was composed of 0.05mol/L ammonium acetate-acetonitrile-0.3mol/L sodium lauryl sulphate (60∶40∶1.5)with a flow rate of 0.5ml/min.RESULTS:The linear range of betahistine was 24～480ng/ml,the recovery rate was 81.75%～87.99%with the intra-day RSD at 0.60%～4.82%and inter-day RSD at 4.78%～12.15%.CONCLUSION:This method developed in the present study is simple,fast,accurate and reproducible,which can be used as the pharmacokinetic study for betahistine in human body.
