Determination of Cilostazol in Human Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中西洛他唑浓度
- Author:
Ping LI
;
Yuguan WEN
- Publication Type:Journal Article
- Keywords:
Cilostazol;
Blood concentration;
HPLC
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of cilostazol in human plasma.METHODS:C18 was the chromatographic column,MeOH-0.03mol/L ammonium acetate (62∶38,V/V)was the mobile phase with a flow rate at 0.8 ml/min and detection wavelength at 257 nm,acetic ether was used as the extraction solvent.RESULTS:Good linear relationship was achieved when the detection concentration range of cilostazol was 10.0~1 500.0 ng/ml(r=0.9 999),the average recovery rates for the high,medium and low concentrations of cilostazol were 99.82%,97.30%and 97.14%,respectively,both the intra-day and inter-day RSD were lower than 6%.CONCLUSION:This method is sensitive,accurate,simple and fast,which can be used as the clinical monitoring of blood concentration of cilostazol and its pharmacokinetics study.