Determination of Quercitrin in Saxifrage by HPLC
- VernacularTitle:高效液相色谱法测定虎耳草中槲皮苷的含量
- Author:
Yimin ZHENG
;
Yanhong YANG
;
Xiuying XU
;
Shanquan FU
- Publication Type:Journal Article
- Keywords:
HPLC;
Saxifrage;
Quercitrin;
Content determination
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the determination of quercitrin in Saxifrage.METHODS:The analysis was performed on C18 column(150mm?4.6mm,5?m),the mobile phase was MeOH-0.2%H3PO4 (45∶55)with a flow rate at 1.0ml/min and wavelength at 350nm under room temperature.RESULTS:There was a good linear relationship when the sample size of quercitrin was at a range of 0.40?g～2.00?g(r=0.9 996),the recovery was 95.33%with RSD at 2.80%.CONCLUSION:This method was simple,stable,fast,and reproducible and without the interference of impurity,which can be used for the content determination of quercitrin in Saxifrage.
