Effect of passive smoking during pregnancy on the expression of TNF-? and CINC in the lungs of neonatal rats
- VernacularTitle:孕鼠被动吸烟对新生大鼠肺组织TNF-?和CINC表达影响的研究
- Author:
Xuxu CAI
;
Yue DU
;
Chunfeng LIU
- Publication Type:Journal Article
- Keywords:
Tobacco smoke pollution;
Tumor necrosis factor;
Interleukin-8;
Rats;
Lung
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of passive smoking during pregnancy on the expression of tumor necrosis factor-? (TNF-?) and cytokine-induced neutrophil chemoattractant (CINC) in the lungs of neonatal rats. Methods Sprague Dawley rats were exposed to sidestream cigarette smoke on the 1, 8 and 15 day after pregnancy for a total of 2 h each day. Those rats who were put into the box without smoke exposure were as control. The neonatal rats were delivered by cesarean section on the 20th day of gestation and birth weight was measured. Reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunoadsorbent assay (ELISA) was performed to evaluate the expression of TNF-?、CINC mRNAs and TNF-? protein. Results The birth weight of neonatal rats born from those mothers who were exposed to sidestream cigarette smoke was significantly lower than that of control gruop(P
