Effect of dexamethasone on the expression of matrix metalloproteinase 2 and type Ⅳcollagen in perinatal rat alveoli
- VernacularTitle:围产期大鼠肺MMP2和Ⅳ型胶原表达及地塞米松对其的影响
- Author:
Changchong LI
;
Shangqin CHEN
;
Chao CHEN
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Pulmonary alveoli;
Rats;
Gelatinase A;
Collagen type Ⅳ
- From:
Chinese Journal of Perinatal Medicine
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the variations of matrix metalloproteinase 2 (MMP2) and type Ⅳcollagen expression in newborn rat alveoli at different stage during pregnancy and the influence of dexamethasone (DXM). Methods Twenty-four Sprague-Dawley (SD) pregnant rats were randomly divided into control group and observe group (12 in each group). Normal saline (0.5 ml) or DXM [0.8 mg/(kg?d)] were injected muscularly to these two groups, respectively, on the 18th,19th and 20th day after conception. All rats were delivered normally at term. The lung tissue were examined in 20 days gestation fetal rats and newborn rats on the 1st, 4th, 10th and 14th day after birth. Immunohistochemical studies were performed for MMP2 and type Ⅳ collagen. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to test the MMP2 mRNA expression. Results The MMP2 expression of day 4 rats (20.14?3.39) was stronger than that of fetal rats lung (16.35?2.36), and decreased in day 10 rats(15.93?1.94). while the general MMP2 expression in the observe group was significantly less than the control(P0.05). The expression of type Ⅳcollagen was stronger than the control group. Conclusion DXM applied in later stage of gestation inhibits the expression of MMP2, reduces the degradation of type Ⅳcollagen, which affect basement membrane and alveoli development.
