Protective effect of propofol on the brain against ischemia-reperfusion injury in rats
- VernacularTitle:异丙酚对大鼠脑缺血再灌注损伤的保护作用
- Author:
Xijiu YE
;
Fangcheng LJ
;
Zongyu TAO
- Publication Type:Journal Article
- Keywords:
Propofol;
Reperfusion injury;
Brain;
Monosaccharide transport proteins
- From:
Chinese Journal of Anesthesiology
1996;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of propofol on the brain against ischemia-reperfusion (I/R) injury. Methods Ninety healthy SD rats weighing 290-310g were randomly divided into 3 groups: Ⅰ propofol group (P) (n =42); Ⅱ normal saline group (NS) (n =42) and Ⅲ sham operation group (S) (n = 6). The animals were anesthetized with intraperitoneal 10% chloral hydrate 0.3 ml?100 g-1. Right external, internal and common carotid arteries were exposed. A nylon thread (0.25 mm in diameter) with rounded tip was inserted at the bifurcation into internal carotid artery and threaded cranially until resistance was felt. The distance from the bifurcation to the tip of the thread was about 17.5-18.5 mm. Middle cerebral artery occlusion (MCAO) was confirmed by ipsilateral Horner's sign and contralateral hemiplegia when the animals were awake after anesthesia. In group P propofol 10 mg?100 g-1 was given intraperitoneally (i .p. ) 10 min before MCAO which was maintained for 1 hour. In group NS, NS was given instead of propofol. In group S the carotid arteries were exposed but MCAO was not performed. Reperfusion was produced by withdrawal of the nylon thread. The animals were killed at 0,2, 5, 11, 23, 71 h and 1 week after reperfusion was started (6 animals at each time-point) . Brains were immediately removed and sliced. The infarct size was analyzed quantitatively by Kontron IBAS 2.5 image auto-analysis system. The glucose transporter-1 (GLUT-1) mRNA was assessed by RT-PCR. The expression of GLUT-1 protein was determined by immuno-histochemistry. Results The infarct size was significantly smaller in P group than in NS group. In P group GLUT-1 mRNA began to increase at Oh, peaked at 23 h and remained higher than normal at 1 week after reperfusion was started and was significantly higher than that in NS group at 0, 2, 5, 11, 23, 71 h and 1 week. The changes in expression of GLUT-1 protein corresponded with the changes in GLUT-1 mRNA.Conclusion Propofol can protect the brain from I/R injury to some extent when given before ischemia. Upregulation of GLUT-1 expression is involved in the mechanism.
