Effects of Insulin on the Gene Expression of Insulin Receptor Substrate-2 in MG-63 Cells
- VernacularTitle:胰岛素对人成骨肉瘤MG-63细胞胰岛素受体底物-2基因表达的影响
- Author:
Qiuxia HUANG
;
Houde ZHOU
;
Eryuan LIAO
- Publication Type:Journal Article
- Keywords:
Insulin;
Insulin receptor substrate-2;
MG-63 cells
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of insulin on the mRNA expression of insulin receptor substrate-2 (IRS-2) in MG-63 cells. Methods Semi-quantitative RT-PCR was used to study the action of insulin on the mRNA expression of IRS-2 in MG-63 cells. Results Insulin regulated the mRNA expression of IRS-2 in a dose- and time-dependent manners in MG-63 cells. Insulin up-regulated the expression of IRS-2 mRNA at 10~ -10～10~ -6mol/L(P
