Determination of Lysophosphatidic Acid by an Enzymatic Cycling Method
- VernacularTitle:循环酶法检测溶血磷脂酸及其临床应用
- Author:
Jiade DENG
;
Yang LI
- Publication Type:Journal Article
- Keywords:
Enzymatic cycling;
Lysophosphatidic acid;
Ovarian carcinoma
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of testing lysophosphatidic acid (LPA) by enzymatic cycling. Methods The detection system was developed on the principle of enzymatic cycling method and characteristic of LPA. The methodology was evaluated and the content of LPA was tested in 20 samples of plasma with ovarian carcinoma. Results The linearity was 18.6?mol/L and the detection limit was 0.03?mol/L. The within-run and between-run coefficient of variations were 5.3% and 7.4%, respectively. The recovery was 100.7% and there was a significant correlation with the liquid chromatography method(Y=0.953X+0.51). The reagent system was stable and different concentrations of bilirubin, hemoglobin, triglyceride, glucose, urea and ascorbate did not affect the measurement for the assay. The content of LPA in 20 samples of plasma with ovarian carcinoma was significantly higher than that in control group (P
