A Study on Tissue Factor Expression and Cell Metastatic Ability in Two Breast Carcinoma Cell Lines
- VernacularTitle:两种不同乳腺癌细胞系组织因子表达与细胞转移力比较研究
- Author:
Yanli GUO
;
Sanjun SONG
- Publication Type:Journal Article
- Keywords:
Tissue factor;
Breast carcinoma;
Metastasis
- From:
Journal of Chinese Physician
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between tissue factor expression and cell metastatic ability in two breast carcinoma cell lines. Methods RT-PCR and flow cytometry(FCM) were used to detect the mRNA and protein expressions of tissue factor(TF) in the breast carcinoma cell lines MCF-7 and MDA-MB231, respectively. The metastatic ability of the two breast carcinoma cell lines was determined using modified Boyden chambers. The data were analyzed by t test. Results The mRNA and protein expression levels of TF in MDA-MB231 were obviously higher than those in MCF-7, and the metastatic ability of MDA-MB231 was stronger than that of MCF-7,which was significantly different between the two cell lines. [WTHZ]Conclusion TF expression was positively related with breast carcinoma metastatic ability, and TF may be a therapeutic target for breast carcinoma.
