Determination of Chrysophanol in Diedazhitong Cataplasm by HPLC
- VernacularTitle:高效液相色谱法测定跌打止痛巴布膏中大黄酚的含量
- Author:
Xiaogen WANG
;
Yanping HUANG
;
Yufan ZOU
- Publication Type:Journal Article
- Keywords:
HPLC;
Diedazhitong cataplasm;
Chrysophanol;
Content
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of chrysophanol in Diedazhitong cataplsam by HPLC.METHODS:The samples were separated on a Kromasasil C 18 ,methol-0.1%phosphoric acid(85∶15)was taken as the mobile phase with a flow rate at1.0ml/min and detection wavelength at254nm,the sample size was10?l.RESULTS:Good linear relationship was achieved when the concentration range of chrysophanol was0.6?g～4?g(r=0.9994);The average recovery of chrysophanol was99.23%(n=5,RSD=2.45%).CONCLUSION:This method is accurate,reliable,and reproducible.
