Simultaneous Determination of2Components in Compound Calcium Gluconate Oral Solution by HPLC
- VernacularTitle:高效液相色谱法同时测定复方葡萄糖酸钙口服溶液中2组分的含量
- Author:
Xiangde SUN
;
Ge GAO
;
Wei QI
- Publication Type:Journal Article
- Keywords:
HPLC;
Calcium lactate;
Calcium gluconate;
Content determination
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the simultaneous determination of calcium lactate and calcium gluconate in compound calcium gluconate oral solution.METHODS:The test was performed on C 18 column with column tem?perature at25℃;the mobile phase was composed of0.025mol/L NaH 2 PO 4 (pH=2.50)with a flow rate of1.0ml/min and sam ple size of20?l;the detection wavelength was210nm.RESULTS:The calibration curves were linear over the range of0.719～46.040mmol/L(r=0.9999)for lactic acid and0.315～20.160mmol/L(r=0.9999)for gluconic acid.The average recovery rates of which were99.0%(RSD=1.65%)and100.5%(RSD=1.36%),respectively.CONCLUSION:This method is fast,simple,accurate,and it can be employed directly without isolation to determine the content of calcium lactate and calcium glu?conate simultaneously and to control the quality of compound calcium gluconate oral solution as well.
