Determination of Peoniflorin in Shenshaogankang Oral Liquid by HPLC
- VernacularTitle:高效液相色谱法测定参芍肝康口服液中芍药苷的含量
- Author:
Hongliang LI
;
Jinhu WU
;
Yuqing XIAO
;
Long LEI
- Publication Type:Journal Article
- Keywords:
HPLC;
Shenshaoankang oral liquid;
Peoniflorin;
Content
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the determination of the content of paeoniflorin in Shenshao?gankang oral liquid.METHODS:Paeoniflorin was separated on Kromasil C 18 ;methanol-0.05mol/L KH 2 PO 4 solution-avantin(60∶173∶4,which was adjusted to pH=4.0with acetic acid)was taken as the mobile phase with a flow rate at1.0ml/min and detection wavelength at230nm.RESULTS:Good linear relationship was achieved when the detection concentration range of peoniflorin was0.027～0.230mg/ml(r=0.9999);The average recovery of peoniflorin was100.97%(RSD=1.28%,n=5). CONCLUSION:The method was simple,rapid,precise and reproducible,which can be used as the quality control of Shenshaogankang oral liquid.
