Content Determination of the Principal Agent in Phenoxymethylpenicillin Potassium Tablets by HPLC
- VernacularTitle:高效液相色谱法测定青霉素V钾片中主药的含量
- Author:
Jianping JIN
- Publication Type:Journal Article
- Keywords:
HPLC;
Phenoxymethylpenicillin potassium;
Content
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the determination of the principal agent in phenoxymethylpenicillin potassium tablets.METHODS:The determination was performed on Diamonsil C 18 column with column temperature at(26?1)℃.The mobile phase consisted of acetonitrile-0.02mol/L sodium acetate(70∶30)with a flow rate at1.0ml/min.The detection wavelength was set at268nm;the sensitivity was0.01AUFS and the sample size was20?l.RESULTS:Good linear correlation with peak area was noted when the detection concentration range was10.0～120.0?g/mL for phenoxymethylpeni?cillin potassium(r=0.9999,n=5).The average recovery was100.2%(RSD=0.42%).CONCLUSION:The method was rapid,simple and accurate and it can be used to determine the content of the principal agent in phenoxymethylpenicillin potas?sium tablets.
