Development of PCR-RFLP for Identification of Eight Mycobacteriai Species
- VernacularTitle:PCR-RFLP检测皮肤分枝杆菌
- Author:
Xiaojie LI
;
Hongsheng WANG
;
Qinxue WU
;
Pangen CUI
;
Xunquan LIU
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Mycobacterium avium;
Mycobacterium;
atypical
- From:
Chinese Journal of Dermatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a PCR-RFLP method for the identification of eight mycobacterial species. Methods PCR was performed targeting the gene encoding 65-kDa heat shock protein which was common to all mycobacteria. Two restriction enzymes, BstE Ⅱ and Hae Ⅲ, were used to digest the PCR products, and specific restriction patterns of different mycobacteria were obtained. Results The specific restriction patterns of different mycobacteria were identical to the data previously reported. Conclusion We could differentiate M. avium, M. intracellulare, M. kansasii, M. tuberculosis, M. scrofulaceum, M. marinum, M. fortuitum and M. chelonae in one experiment by PCR-RFLP.
