Effects of Recombinant Chimeric Toxin Dsg3EC_(1-2)PE40 on T and B Lymphocytes from Pemphigus Vulgaris
- VernacularTitle:重组嵌合毒素Dsg3EC_(1-2)PE40对寻常型天疱疮患者T、B淋巴细胞的影响
- Author:
Zhifang ZHAI
;
Qingchun DIAO
;
Fei HAO
;
Dabin SHEN
;
Baiyu ZHONG
;
Shuqian TANG
- Publication Type:Journal Article
- Keywords:
Pemphigus;
Immunotoxins;
Desmoglein
- From:
Chinese Journal of Dermatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of recombinant chimeric toxin Dsg3EC_(1-2)PE40 on T and B cells from PV patients. Methods The recombinant protein Dsg3EC_(1-2)PE40 was expressed on BL21TrxB (DE3) cells, then identified and purified. ELISPOT assay was used to detect and quantitate autoantibody-producing B cells in different concentrations of recombinant chimeric toxin, and MTT assay and ~3H-TdR assay to observe the metabolism and proliferation of T cells from PV patients in vitro. Results The purity of expressed protein Dsg3EC_(1-2)PE40 was up to 80%. The number of anti-Dsg3 antibody-producing B cells in PBMC from PV patients decreased by 40% with treatment of Dsg3EC_(1-2)PE40, which was significantly lower (P
