Effect of Aloin on Inducible Nitric Oxide Synthase and Nuclear Factor Kappa B Synthesis of HaCat Cells Irradiated by Ultraviolet B
- VernacularTitle:芦荟甙对中波紫外线辐射HaCaT细胞表达诱导型一氧化氮合酶及核因子?B的影响
- Author:
Xiuzu SONG
;
Aie XU
;
Zhigang BI
- Publication Type:Journal Article
- Keywords:
Ultraviolet rays;
Cells, cultured;
Nitric-oxide synthase;
NF-kappa B;
Aloin
- From:
Chinese Journal of Dermatology
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of aloin on inducible nitric oxide synthase (iNOS) and nuclear factor kappa B (NF-kB) synthesis of HaCat cells induced by ultraviolet B (UVB) irradiation. Methods The proliferation of HaCat cells was measured by MTT method. iNOS mRNA was detected by reverse transcriptase polymerase chain reaction (RT-PCR). NO production was assessed by spectrophotometric method, and expression of NF-kB P65 was measured by immunofluorescent staining. Results After irradiation with 30 mJ/cm~2 of UVB, proliferation of HaCat cells was decreased, and NO generation and iNOS mRNA synthesis in HaCat cells were increased. UVB irradiation could also activate the expression of NF-kB P65 and promote its translocation into nucleus. NO generation and iNOS mRNA synthesis were markedly down-regulated in a dose-dependent manner by pre-treatment with different concentrations of aloin. The activation of NF-kB P65 was inhibited while the proliferation of HaCat cells was increased. All the difference reached statistical significance (P
