Assay of Adriamycin in Endolymph of Guinea Pig Cochlea By High Performance Liquid Chromatography
- VernacularTitle:豚鼠耳蜗内淋巴液阿霉素含量高效液相色谱法测定方法的建立
- Author:
Yaguang ZHOU
;
Xueyuan ZHANG
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography(HPLC);
Adriamycin;
Cochlea;
Endolymph
- From:
Journal of Audiology and Speech Pathology
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To create a method to assay adriamycin in endolymph of guinea pig cochlea,and provide methodology background to study the accumulation of ototoxicity substance in inner ear.Methods 12 normal guinea pig were killed quickly after 24 hours following adriamycin injection.The concentrate of adriamycin in endolymph of guinea pig cochlea was assayed by high performance liquid chromatography(HPLC).Results The linear relation of concentration of adriamycin in endolymph was well when the concentration was from 2.56 ng/mL to 1398.00 ng/mL.Correlation coefficient of linearity was 0.999 (n=10).The percentage of retrieve for adriamycin in endolymph was 96.3%.Relative standard deviation (RSD) was 2.61%.The lowest limitation was 1.9 ng/ml .Conclusion The sensibility of HPLC is high,and it can accurately detect the concentration of adriamycin in endolymph of guinea pig cochlea,especially the adriamycin is administrated in vein.
