Determination of Activity of Drug-metabolizing Enzyme CYP1A2 in Livers of Healthy Adults by Caffeine Probe Method
- VernacularTitle:咖啡因探针法测定正常人肝脏药物代谢酶CYP1A2活性
- Author:
Jian ZHANG
;
Xiangqian PENG
;
Jun LI
- Publication Type:Journal Article
- Keywords:
Caffeine;
Metabolite;
HPLC;
Cytochrome P4501A2;
Probe method
- From:
China Pharmacy
2005;0(16):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of4major caffeine metabolites and to discuss the significance of which in the evaluation of the activity of drug-metabolizing enzyme CYP1A2.METHODS:The caffeine metabolites in the urine like5-acetylamino-6-formamido-3-methyluric acid(AFMU),1-methyluric acid(1U),1-methylxanthine(1X)and1,7-dimethyluricacid(17U)were determined by RP-HPLC gradient elution method,the ratios of metabolins(AFMU+1X+1U)/17U was calculated,the frequency distribution histogram was drawn and the activity of CYP1A2was evaluated.RESULTS:The mean value of the ratio of the metabolins in the subjects was4.27,which was in normal distribution.CONCLUSION:The method is simple,accurate and rapid,which is suitable for the determination of caffeine metabolites in urine and the study of the activities of CYP1A2.
