The effect of nerve growth factor on the proliferation of human cholangiocarcinoma cells
- VernacularTitle:神经生长因子对人胆管癌细胞增殖作用的影响
- Author:
Wei GUO
;
Shengquan ZOU
- Publication Type:Journal Article
- Keywords:
Bile Duct Neoplasms;
Cell Protiferation;
Tumor Cells,Cultured;
Nerve Growth Factor
- From:
Chinese Journal of General Surgery
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nerve growth factor(CNF) on the proliferative ability of (human) cholangiocarcinoma cell strain QBC939. Methods After construction of a full-length human ?-NGF expression vector, human cholangiocarcinoma cell QBC939 was transfected with pcDNA3.0-NGF. Western blot assays were used to detect the expression of ?-NGF. Subsequently. In vitro proliferation before and after transfection of cells was analyzed by MTT assays. Results Western blot assay indicated that ?-NGF could be stably expressed by transfected QBC939 cells, and compared with controls, the differences were significant (P
