Apoptosis of colorectal cancer cells transfected with human mutant p27
- VernacularTitle:人突变p27基因转染大肠癌细胞凋亡的实验研究
- Author:
Jun CHEN
;
Shaoyong XU
;
Ling XIONG
;
Jianing WANG
;
Yongzhang HUANG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Genes, p27mt;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the inducing effect of human mutant p27 gene on apoptosis of the colorectal cancer cells. METHODS: The recombinant adenovirus Ad-p27mt was constructed to infect the colorectal cancer cell SW480. The inducing effect of Ad-p27mt on apoptosis in colorectal cancer cells was measured by flow cytometry, DNA fragment analysis and TUNEL method. RESULTS: Ad-p27mt was successfully constructed. When the multiplicity of infection (MOI) was ≥50, the infection efficiency reached 100%. After 24 h of infection, there was an apoptotic hypodiploid peak observed by flow cytometry before G_1 and there were apoptotic characteristic bands in the DNA electrophoresis. The apoptotic index detected by TUNEL method was 82.6?3.2 (Ad-p27mt group) and 5.0?3.5 (control group), respectively, the difference of which was significant (P
