Effect of Shenmai injection on cardiomyocyte apoptosis after acute anoxia-reoxygenation
- VernacularTitle:参麦注射液对急性缺氧-复氧心肌细胞凋亡的影响
- Author:
Ran HAO
;
Yanjing CHEN
;
Qifu HUANG
- Publication Type:Journal Article
- Keywords:
Shenmai injection;
Cardiomyocytes;
Anoxia-reoxygenation;
Apoptosis;
Calcium overload
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of Shenmai injection on cardiomyocytes apoptosis after acute anoxia-reoxygenation (A/R) and the possible mechanism. METHODS: In this experiment, cultured cardiomyocytes isolated from neonatal rat were used. Model of myocardial anoxia-reoxygenation injury was produced by depriving oxygen for 5 min and then restoring oxygen for 15 min. The apoptotic cells was detected by flow cytometry to detect labbled Annexin V-FITC/PI. The intracellular calcium level was observed by laser scanning confocal microscopy markered Fluo-3/AM. RESULTS: In anoxia-reoxygenation group, the percentage of apoptotic cells and fluorescent intensity of intracellular calcium were both prominently higher than those in control group (P
