Overexpression of ?-synuclein in SH-SY5Y cells in vitro leads to oxidative stress
- VernacularTitle:?-突触核蛋白在体外培养SH-SY5Y细胞内过表达可导致氧应激
- Author:
Yuxin ZHANG
;
Ziming ZHANG
;
Hui YANG
;
Qing CAI
;
Qiang LU
;
Biao CHEN
;
Qunyuan XU
- Publication Type:Journal Article
- Keywords:
Synuclein;
Stress;
Parkinson disease;
SH-SY5Y cells
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To observe the influence of overexpression of ?-synuclein on the cultured SH-SY5Y cells. METHODS: The plasmid of ?-synuclein-pcDNA_3 was transfected into SH-SY5Y cells with LipofectAMINE. The expression of ?-synuclein was determined by anti-?-synuclein immunocytochemistry. The intracellular reactive oxygen species was determined with 2', 7'-dichlorofluorescein diacetate (DCF-DA) by using a FACSCAN flow cytometer and fluorescent microscope. The intracellular content of reduced GSH was detected with glutathione assay kit by spectrophotometer. RESULTS: The ?-synuclein was expressed in cultured SH-SY5Y cells transfected with the plasmid of ?-synuclein-pcDNA_3. The DCF loading analysis and the intracellular level of reduced GSH suggested that the transfected cells were under oxidative stress. CONCLUSION: Overexpression and accumulation of ?-synuclein in SH-SY5Y cells increase intracellular reactive oxygen species levels, it is suggested therefore that the ?-synuclein does play an important role in the pathogenesis of Parkinson's disease.
