Effects of the dominant-negative I?B? plasmid on the expression of NF-?B and cyclooxygenase-2 in pancreatic carcinoma
- VernacularTitle:显性失活I?B?质粒对胰腺癌PC-3细胞株核因子-?B和环氧合酶-2表达的影响
- Author:
Chuangao XIE
;
Shumei WEI
;
Xuanfu XU
;
Xingpeng WANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
NF-kappa B;
Plasmids;
Cyclooxygenase-2
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of the dominant-negative I?B? plasmid on the expression of NF-?B and cyclooxygenase-2 (COX-2) after its being transfected into pancreatic carcinoma PC-3 cell line. METHODS: The expression of NF-?B and COX-2 in PC-3 cell line was confirmed by immunohistochemistry. The effects of dominant-negative I?B? plasmid transfection on the expression of NF-?B and COX-2 were studied by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. RESULTS: Both NF-?B and COX-2 were expressed in pancreatic carcinoma PC-3 cell line, and the expression of NF-?B and COX-2 were down-regulated in a certain time-independent manner after dominant-negative I?B? plasmid transfection. CONCLUSIONS: The NF-?B and COX-2 are expressed in pancreatic carcinoma PC-3 cell lines. The expression of NF-?B and COX-2 are inhibited by dominant-negative I?B? plasmid, while NF-?B is likely to play an important role in regulating the expression of COX-2.
