Overexpression of ErbB2 promotes growth and invasion in MCF-7 cells in vitro
- VernacularTitle:过量表达ErbB2促进MCF-7细胞体外生长和侵袭
- Author:
Baogang PENG
;
Qiang HE
;
Lijian LIANG
- Publication Type:Journal Article
- Keywords:
Genes, ErbB2;
MCF-7 cells;
Cell division;
Invasiveness
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of ErbB2 overexpression on growth and invasiveness in cultured MCF-7 cell line. METHODS: Retrovirus containing ErbB2 gene was transfected into MCF-7 cells and ErbB2 expression was detected by Western blotting. Proliferation and invasive assays were carried out. Cells overexpressed ErbB2 and its control, AP2, were used in the experiment. RESULTS: ErbB2 was overexpressed in MCF-7 cells after transfection. In vitro, cells overexpressed ErbB2 showed highly proliferated and highly invasive characteristics compared to the control cells. CONCLUSION: Overexpression of ErbB2 promotes cell proliferation and enhances invasiveness in MCF-7 cells. Inhibition of signaling induced by ErbB2 might be a novel strategy for the therapeutics of cancer with ErbB2 overexpression.
