Effect of glutamine on heat shock protein-70 and tumor necrosis factor-? expession in endotoxemic rats
- VernacularTitle:谷氨酰胺对脂多糖血症大鼠热休克蛋白70和肿瘤坏死因子-?表达的影响
- Author:
Min ZHAO
;
Xing MENG
- Publication Type:Journal Article
- Keywords:
Glutamine;
Lipopolysaccharides;
Heat shock proteins 70;
Tumor necrosis factor
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the protective effect of glutamine (Gln) against endotoxemia by observing the effect of glutamine on heat shock proteins (HSPs) and tumor necrosis factor-? (TNF-?) in endotoxemic rats. METHODS: The rats were randomly divided into 3 groups, lipopolysaccharide group (LPS), glutamine-treated group (Gln) and control group (C). The blood was drawn from lateral tail vein for analysis of cytokine levels at 0, 2, 4 and 6 h post-lipopolysaccharide (LPS) challenge. TNF-? was measured by radioimmunity assay. Multiple tissues were harvested from the rats, and HSP70 was detected by immunohistochemistry. At the same time, lung, liver, and ileum tissue section were stained with hematoxylin and eosin. RESULTS: Gln treatment resulted in marked attenuation of TNF-? expression at 2 h post-LPS injection (P
