Changes of tear film stability after rebuilding ocular surface with corneal stem cells
- VernacularTitle:角膜干细胞重建眼表后泪膜稳定性改变的试验研究
- Author:
Hong ZHANG
;
Bing WANG
;
Yanfeng YI
- Publication Type:Journal Article
- Keywords:
Stem cells;
Cornea
- From:
Chinese Journal of Pathophysiology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the physiological function changes of the tears film after rebuilding ocular surface with corneal stem cells, and to discuss the validity and the estimate system of rebuilding ocular surface with the corneal stem cells. METHODS: The male New Zealand rabbits were used to establish the alkali burning model in the right eye. The corneal stem cells of the left eye were cultured on the amniotic membrane in vivo, and then transplanted to the right eye. Furthermore, the physiological function changes of the tear film were examined. RESULTS: Compared to the before alkali burning, the ocular surface cell morphology was similar after rebuilding ocular surface with the corneal stem cells, which were cultured on the amniotic membrane in vivo; The tear film breakup time test showed the a remarkable difference between after and before the alkali burning, but the cell modality after rebuilding had no remarkable difference compared to that before the alkali burning. CONCLUSIONS: It's an effective method to rebuild the ocular surface with the corneal stem cells in vivo, the cell framework and the physiological function of the tears film recover well after rebuilding. It may be a validity estimate system of rebuilding ocular surface to analyze framework and configuration of the ocular surface and test the tear film breakup time.
