Construction of Antisense GA cDNA Recombinant Plasmid and Its Effect on the Growth of Colon Cancer Cells
- VernacularTitle:反义GAcDNA重组质粒的构建及其对结肠癌细胞增殖的影响
- Author:
Yibin LI
;
Juntao YANG
;
Hongming LIU
- Publication Type:Journal Article
- Keywords:
Gene recombination;
Plasmid;
Colon cancer;
Growth
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the recombinant plasmid carrying antisense GA cDNA, and evaluate the effect of antisense GA gene on the biological characteristics of colon cancer cells. Methods The recombinant plasmid pcDNA3.0/GA containing antisense GA gene was constructed by gene recombination technique. The recombinant plasmid was transfected into colon cancer cells by lipofectamine. The effect of antisene GA gene on the biological characteristics of colon cancer cell line DH52 was evaluated by MTT and electron microscopy. Results The recombinant plasmid pcDNA3.0/GA was successfully constructed. The growth rate of colon cancer cells decreased, and microstructure of the cells appeared typical apoptotic changes after transfection. Conclusion Antisense GA gene could inhibit the growth of colon cancer cells possibly by suppressing GA gene expression and decreasing GA activity in the colon cancer cells.
