Diagnosis of Pancreatic Cancer by Detecting K-ras Gene Mutations
- VernacularTitle:应用K-ras基因突变诊断胰腺癌的探讨
- Author:
Guangjun SUO
;
Dianxu FENG
;
Hui ZHANG
- Publication Type:Journal Article
- Keywords:
K-ras;
Pancreatic cancer;
CED-RFLP/PCR;
Plasma
- From:
Journal of Chinese Physician
2001;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect plasma K-ras gene mutation by using CED-RFLP/PCR to diagnose pancreatic cancer. Methods CED-RFLP/PCR technique was used to detect K-ras gene mutation in the plasma specimens of pancreatic cancer patients, patients with benign pancreatic diseases and healthy subjects. Results In pancreatic cancer patients the positive rate of plasma K-ras gene mutation was 73%, without false positivity, and higher than that in the pancreatic juice and duodenal juice, but lower than that in the fine-needle aspirates. Plasma K-ras gene mutation was not found in patients with benign pancreatic disease and healthy subjects. Conclusion The detection of plasma K-ras gene mutation by CED-RFLP/PCR is simple and effective, and could avoid the faults of other detection methods. It is helpful for the diagnosis and identification of pancreatic cancer.
