Expression of MCP-1 and MIP-2 mRNA in Vagina of Murine Model of Vulvovaginal Candidiasis
- VernacularTitle:外阴阴道念珠菌病小鼠模型阴道组织趋化因子MCP-1和MIP-2 mRNA水平测定
- Author:
Jingjun ZHAO
;
Cuihong LIAN
;
Donghong ZHAO
;
Chaojun LI
;
Weida LIU
- Publication Type:Journal Article
- Keywords:
Candidiasis,vulvovaginal;
Animal testing alternatives;
Monocyte chemoattractant protein-1;
Macrophage inflammatory proteins
- From:
Chinese Journal of Dermatology
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the kinetic expression level of chemokines (MCP-1 and MIP-2) in vagina of a murine model of vulvovaginal candidiasis (VVC).Methods The estrogen-treated murine model of VVC was set up.Vaginal specimens were obtained in different duration after inoculation of C.albicans intravaginally.Semi-quantitative RT-PCR was applied to determine MCP-1 and MIP-2 mRNA levels in these tissues.Results Compared with the control mice treated with olive oil,persistent growth of C.albicans was found from the 2nd day to 21st day after inoculation in estrogen-treated mice.Significantly higher levels of MCP-1 mRNA were observed in vaginal tissues in infected estrogen-treated mice than those in other 2 groups,infected but non estrogen-treated mice and estrogen-treated but uninfected mice.The high level of MCP-1 mRNA maintained from the 4th day to 21st day in infected estrogen-treated mice.It was also found that levels of MIP-2 mRNA were significantly higher in the vagina in the 2nd day in 3 groups of mice than those in naive mice,however,no significant difference was shown among 3 groups throughout the study period.Conclusion High level of MCP-1,rather than MIP-2,may be associated with susceptibility to VVC.
