In vitro study on the effect of PKC-NF-?B signal transduction pathway on preeclamptic umbilical sera-induced collagen expression in HUASMC
- VernacularTitle:PKC-NF-?B信号传导对子前期患者脐血清诱导脐动脉平滑肌细胞胶原mRNA表达影响的体外研究
- Author:
Rongzhen JIANG
;
Hanping CHEN
- Publication Type:Journal Article
- Keywords:
Pre-eclampsia;
Polymyxin B;
NF-kappa B;
Umbilical arteries;
Muscle, smooth, vascular;
Collagen
- From:
Chinese Journal of Perinatal Medicine
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of protein kinase C-nuclear factor-kappa B (PKC-NF-?B) signal transduction pathway on the umbilical sera-induced human umbilical artery smooth muscle cell (HUASMC) proliferation and the expression of precollagen Ⅰ, and Ⅲ mRNA in preeclamptic women. Methods HUASMC from normal pregnancy were cultured and generated. After 30 minutes of incubation with or without Polymyxin B (PMB), the umbilical serum from the preeclamptic women was added. Two hours later, the expression of Ⅰ-?B and NF-?B were detected by Western blot. After 48 h, the activity of HUASMC was evaluated by MTT, the cell cycle by flow cytomerty and the expression of precollagenⅠ,ⅢⅢ and bcl-3 mRNA by reverse transcription-polymerase chain reaction(RT-PCR). Results Ⅰ-?B level (0.3765?0.0321) and the percentage of G_0+G_1 phase cells (62.53?2.85)% in preeclamptic group were significantly lower than those in control group [(0.7857? 0.0296),(78.66?3.25)%, P
