Hypoxia upregulates CD73 expression in mouse microvascular endothelial cells
- VernacularTitle:低氧增强小鼠脑微血管内皮细胞CD73表达
- Author:
Xiaobo LI
;
Ping ZHOU
;
Fengdi ZHAO
;
Lianhua YIN
- Publication Type:Journal Article
- Keywords:
Hypoxia;
5’-nucleotidase;
Adenosine;
Endothelium, vascular
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of hypoxia on CD73 expression in mouse microvascular endothelial cell line bEnd.3. METHODS: ① bEnd.3 cells were exposed to different periods of hypoxia. ② Concentration of LDH released by bEnd.3 cells into the culture medium was detected. ③ Surface CD73 activity in bEnd.3 cells was measured by HPLC according to the conversion of E-AMP to E-ADO. ④ CD73 mRNA expression were analyzed by semiquantitative RT-PCR. ⑤ Cell surface proteins were biotinylated and CD73 was detected by avidin blots of immunoprecipitation with mAb TY23. RESULTS: ① bEnd.3 cells exposed to hypoxia for 24 h demonstrated a significant increase in LDH release (P
