Effects of homocysteine on number and activity of endothelial progenitor cells from peripheral blood
- VernacularTitle:同型半胱氨酸对外周血内皮祖细胞数量和功能的影响
- Author:
Xingxiang WANG
;
Yunpeng SHANG
;
Junhui ZHU
;
Junzhu CHEN
;
Jianhua ZHU
;
Qianmin TAO
;
Xiaogang GUO
;
Zhankun WANG
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Endothelial cells;
Homocysteine;
Microscopy, confocal;
Coronary disease
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate whether homocysteine (Hcy) has influences on endothelial progenitor cell (EPCs) number and activity from peripheral blood. METHODS: Total mononuclear cells (MNCs) were plated on fibronectin-coated culture dishes and cultured for 7 days, and then attached cells were stimulated with Hcy or vehicle control for 6 h, 12 h, 24 h and 48 h. The adhesion, proliferation, migration and in vitro vasculogenesis activity of EPCs were assayed, respectively. RESULTS: Incubation of isolated human MNCs with Hcy dose and time-dependently decreased the number of EPCs with maximum at 200 (?mol/L) for 24 hours (35.7?6.7 vs 62.5?10.6, P
