Role of TGF-?_1 and its signaling transduction molecule Smad4 in the development of glomerulosclerosis
- VernacularTitle:转化生长因子?及其信号转导分子Smad4在肾小球硬化中的作用研究
- Author:
Wei SHI
;
Chaosheng HE
;
Shuangxin LIU
;
Xinling LIANG
;
Zhiming YE
;
Yongzheng LIANG
;
Xiangming HU
;
Zhaojie WANG
- Publication Type:Journal Article
- Keywords:
Transforming growth factor beta;
Smad4;
Glomerulonephritis;
Signal transduction
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the role of TGF-?_1 and signaling transduction molecule, Smad4, in the development of glomerulosclerosis. METHODS: Expression levels of TGF-?_1, Smad4, collagen Ⅰ proteins were evaluated by immunohistochemistry in renal biopsies from 38 cases with a spectrum of glomerulonephritis, and compared with 20 normal kidney tissue with image analysis system. After stimulation with TGF-?_1, expressions of endogenous Smad4 and collagen Ⅰ mRNA and proteins and its modulation by TGF?_1 were evaluated by RT-PCR and Western blotting analyses in cultured human mesangial cells. RESULTS: All types of proliferative and sclerotized glomerulonephritis showed an increased expression of TGF-?_1, Smad4 and collagen Ⅰ ompared with the 20 normal kidney tissue in glomerular (P
