Effect of the NF-?B inhibitor pyrrolidine dithiocarbamate on the apoptosis and proliferation of mesangial cells in the rat with anti-thymocyte serum nephritis
- VernacularTitle:NF-?B抑制剂(PDTC)对大鼠抗胸腺细胞血清性肾炎系膜细胞凋亡、坏死及增生的影响
- Author:
Jinghua XU
;
Yingwei WANG
;
Juan XU
;
Yiqin WU
;
Jianxia TONG
;
Bin SUN
- Publication Type:Journal Article
- Keywords:
Pyrrolidines;
NF-kappa B;
Nephritis;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the effect of pyrrolidine dithiocarbamate (PDTC), the specific inhibitor of NF-?B, on anti-thymocyte serum nephritis (ATSN) in rats. METHODS: The rat model of ATSN was reproduced with rabbit anti-thymocyte serum (ATS). The rats were divided into ATSN group, ATSN+PDTC group and control group. The expression of NF-?B p65 and the apoptosis, lysis as well as proliferation of mesangial cells (MC) were examined by immunohistochemical staining, Tdt-mediated X-dUTP nick end labeling (TUNEL), light microscope and electron microscope at 40 minutes, 24 hours and 7 days after injection of ATS or normal serum. RESULTS: The expression of glomerular NF-?B p65 in the ATSN group was observed with significant difference compared to controls at 40 min (P
