Inhibition of tyrosinase gene expression by antisense nucleotide in murine B16 melanoma cells
- VernacularTitle:反义核酸抑制B16黑色素瘤细胞tyr基因的表达
- Author:
Qiongmei JI
;
Zhenyu ZHU
;
Xiaohua WANG
;
Dongfang ZHANG
;
Haitao ZHANG
;
Xiuying LI
;
Jianquan MA
- Publication Type:Journal Article
- Keywords:
Nucleotide, antisense;
Melanoma;
Monophenol monooxygenase;
Genes, tyr
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To inhibit the expression of tyrosinase gene in murine B16 melanoma cells by antisense nucleotide. METHODS: The antisense recombinant pcDNA3.1(-)-tyr was constructed and was used to infect murine B16 melanoma cells for expression of tyr antisense nucleotide. The effect of antisense nucleotide of tyr on the expression of tyr gene was detected by determination of the activity of tyrosinase and of the production of melanin, Dopa staining and electronic microscope. RESULTS: The tyr antisense recombinant was successfully constructed and injected into murine B16 melanoma cell. The activity of tyrosinase in B16 cells infected with pcDNA3.1 (-)-tyr decreased to 0.0498?0.0036, compared to the tyrosinase activity of 0.0916?0.0132 in the control cells without treatment (P
