Experiment research of anti-PG cell metastasis and mechanism of a new Chinese herb Qcimum basilicum polysaccharide
- VernacularTitle:体外罗勒多糖抗人高转移肺癌细胞侵袭转移作用及机制探讨
- Author:
Xun QU
;
Guangjuan ZHENG
;
Deshan LIU
;
Meixiang YANG
;
Jinbo FENG
;
Shi YAN
- Publication Type:Journal Article
- Keywords:
Qcimum basilicum polysaccharide;
Neoplasm metastasis;
Lung neoplasms
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: In this paper, we studied the efficiencies and the mechanisms of a new Chinese herb Qcimum basilicum polysaccharide (BP) on PG cell metastasis in vitro. METHODS: The number of tumor cells going through matrigel was assayed and used to represent the ability of the invasion and migration of PG cells. Using Scrape-loading and dye transfer (SLDT) technique, the efficiencies of BP on recovering PG cell gap junction -mediated intercellular communication (GJIC) was measured. The expressions of c-myc, nm23-H1 and Tiam-1 genes mRNA in PG cells treated with BP were determined by RT-PCR. RESULTS: Compared with the control, the action of invasion and migration of PG cells were decreased after treated with BP (P
