Effect of compound poria prescription on proliferation and differentiation and expression of PPAR? mRNA in rat preadipocytes
- VernacularTitle:复方茯苓制剂对大鼠前脂肪细胞增殖分化以及过氧化物酶体增生物激活受体?mRNA表达的影响
- Author:
Lei LIU
;
Shengyuan LIU
- Publication Type:Journal Article
- Keywords:
Rats;
Adipocytes;
Obesity;
Drugs, Chinese herbal;
Peroxisome proliferators
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of herbal compound poria prescription (CPP) on proliferation and differentiation in primary rat preadipocytes, and to explore the mechanism of this process. METHODS: Primary cultured rat preadipocyte proliferation was determined by cell counting and MTT spectrophotometry. Cell differentiation was determined by Oil Red O staining, and the expression of PPAR? mRNA was determined by RT-PCR. RESULTS: CPP inhibited the proliferation and differentiation of primary rat preadipocytes in a dose-dependent manner, and it also inhibited the expression of PPAR? mRNA. CONCLUSION: CPP inhibits the proliferation of primary rat preadipocytes and inhibits the preadipocyte differentiation by suppressing the expression of PPAR? mRNA.
