The mitochondrial and structural protein changes in dexamethasone-induced mouse thymocyte apoptosis
- VernacularTitle:地塞米松介导胸腺细胞凋亡过程中线粒体和细胞结构蛋白的变化
- Author:
Tong WANG
;
Yaoying ZENG
;
Jingxian ZHAO
;
Yi LIN
;
Peiyan LIANG
- Publication Type:Journal Article
- Keywords:
Dexamethasone;
Thymocytes;
Cellular structural protein;
Apoptosis;
Mitochondria
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study mitochondrial mass and structural protein changes in dexamethasone (DEX)-mediated mouse thymocyte apoptosis process. METHODS: DEX-induced mouse thymocyte apoptosis model was established. Annexin V-FITC/PI double staining was used to identify apoptotic and necrotic cells by flowcytometry, JC-1 staining was adopted to test mitochondrial membrane potential (△?_m), and cellular structural protein changes were studied with CFDA-SE staining. RESULTS: By 1?10~(-6) mol/L DEX stimulation, the apoptotic rate was 51.25%?5.51% and had significantly difference from control group (12.03%?2.00%); the necrotic rate in DEX group was 30.25%?3.67% and also had significantly difference from control group (10.11%?1.11%, P
