Effects of Ubiquitin-proteasome Inhibitor MG-132on Apoptosis and Survivin Expression of Esophageal Carcinoma Cells
- VernacularTitle:泛素-蛋白酶体抑制剂MG-132对食管癌细胞凋亡和生存素表达的影响
- Author:
Weiguo ZHANG
;
Guojian XIE
;
Qibin WANG
;
Xiaohu WANG
;
Qingming WU
;
Qiang WANG
;
Qiang TONG
;
Shengbao LI
- Publication Type:Journal Article
- Keywords:
Ubiquitin-proteasome;
Esophageal carcinoma;
Apoptosis;
Survivin
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate effects of ubiquitin-proteasome inhibitor MG-132on apoptosis and survivin expression of esophageal carcinoma cells.METHODS:The esophageal carcinoma cells Eca9706were treated with MG-132,the growth inhibitory rate was determined with MTT assay,apoptosis was detected by flow cytometry,expression of survivin was detected by immunocytochemical technique.RESULTS:MG-132had obvious inhibitory effects on the growth of gastric car?cinoma cells,IC 50 of24hrs,48hrs,72hrs and96hrs were120.2,18.1,—12.2,and—16.9?mol/L respectively;Treated with5.0?mol/L MG-132for24hrs,48hrs,72hrs and96hrs,apoptotic rates of cells were(3.1?0.4)%、(31.7?3.5)%、(50.4?4.8)%and(66.6?6.2)%respectively;Expression of survivin was high in esophageal carcinoma cells and it was decreased in cells treated with MG-132.CONCLUSIONS:MG-132can significantly inhibit the proliferation of esophageal carcinoma cells and induce apoptosis,which might be associated with down-regulated expression of survivin.
