Simultaneous Determination of Plasma Rabeprazole and It's Metabolites by RP-HPLC
- VernacularTitle:反相高效液相色谱法同时测定人血浆中雷贝拉唑及其代谢产物
- Author:
Chunyan NIU
- Publication Type:Journal Article
- Keywords:
Rabeprazole;
Thioether-rabeprazole;
Demethylated thioether-rabeprazole;
RP-HPLC;
Plasma
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish RP-HPLC method for the simultaneous determination content of rabeprazole(RAB)and its two metabolites thioether-rabeprazole(TE)and demethylated thioether-rabeprazole(DMTE)in human plasma.METHODS:The plasma samples were extracted with ethyl acetate;The Diamonsil C 18 reversed-phase column was used as analytical column,the mobile phase was consisted of methanol-water-glacial acetic acid-triethylamine(40∶60∶0.4∶0.1)with a flow rate at1.0ml/min,the ultraviolet detection wavelength was288nm.RESULTS:Good separation of chromatographic peaks of RAB,TE and DMTE were manifested.The mean recovery of which were110.40%,87.28%and98.21%respectively.CONCLUSION:The method was sensitive and accurate,and it meets the requirements for the study of pharmacokinetic of rabeprazole.
