Content Determination of the 3 Constituents in Jiangzhiyin by HPLC Simultaneous
- VernacularTitle:高效液相色谱法同时测定降脂饮中3组分的含量
- Author:
Honglan ZHONG
;
Li XIE
;
Tao TAO
- Publication Type:Journal Article
- Keywords:
Jiangzhiyin;
Rhein;
Emodin;
Chrysophanol;
HPLC
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of the effective constituents rhein,emodin and chrysophanol in jiangzhiyin by HPLC.METHODS:SHIMADU VP-ODS column was used as analytical column,methanol-0.2%phosphoric acid(80∶20)as mobile phase,the flow rate was set at1ml/min,the detection wavelength at225nm,column temperature at room temperature.RESULTS:The linear ranges of detected rhein,emodin and chrysophanol in jiangzhiyin were0.015～0.15?g(r=0.9996),0.014～0.14?g(r=0.9998)and0.0085～0.085?g(r=0.9993)respectively;The average recoveries were99.26%(RSD=2.00%),98.72%(RSD=1.80%)and99.76%(RSD=2.3%)respectively.CONCLUSION:The determination results are stable and accurate,and the method is feasible and reliable,which can be used for the quality control of jiangzhiyin.
