Contents Determination of 2 Components in Hadro-chlorine Gutta by HPLC
- VernacularTitle:高效液相色谱法同时测定强氯滴眼液中2组分的含量
- Author:
Ming CHANG
;
Jing LI
;
Shufang LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Chloramphenicol;
Prednisolone acetum;
Content determination
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the HPLC method for the simultaneous content determination of chloramphenicol and prednisolone acetum in hadro-chlorine gutta.METHODS:The chromatographic column was HiQ sil C 18 ,the methanol-water-glacial acetic acid(70∶30∶0.04)served as mobile phase,and the detecting wavelength was278nm,the flow rate was0.8ml/min and the column temperature was30℃,the sample size was10?l.RESULTS:A satisfactory linear relationship be?tween was found when the concentration determination ranges of chloramphenicol and prednisolone acetum at22.36～89.44?g/ml and25.00～100.00?g/ml respectively;The average recoveries for chloramphenicol and prednisolone acetum were99.65%(RSD=0.88%)and99.57%(RSD=1.02)respectively.CONCLUSION:The method was simple,accurate and good in repro-ducibility,which can be used for the simultaneous contents determination of 2 components in hadro-chlorine gutta.
