Technical improvement of "two-cuff method" of orthotopic liver transplantation in rats
- VernacularTitle:“二袖套法”大鼠原位肝移植的技术改进
- Author:
Tao LI
;
Huamei TANG
;
Xing SUN
;
Zhihai PENG
- Publication Type:Journal Article
- Keywords:
Liver Transplantation;
Graft Rejection;
Diseuse Models,Animal
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate an improved technique of orthotopic liver transplantation with the ("two-cuff method") in rats, and to observe allograft rejection after transplantation. Methods30 Wistar rats were grafted with livers of SD rats and 30 SD rats were grafted with livers of SD rats by using a modified "two-cuff technique" and examined for postoperative rejection. ResultsThe anhepatic period was about 15 minutes. The 24-hour survival rate was 100%. The Wistar rats grafted with SD livers died 8 to 15 days (after) liver transplantation, and the histopathologic examination showed various degrees of allograft rejection. The SD rats grafted with livers of SD rats had a survival rate of 97% at 3 weeks postoperatively. ConclusionsThe modified "two-cuff technique" can effectively reduce operative time, decrease postoperative (complications) and increase the survival rate of othortopic liver transplantation in rats. The transplantation model of Wistar rats grafted with livers of SD rats can be used to study allograft rejection.
