TGF-?_1 gene modified dendritic cells induced tolerance in islet xenotransplantation
- VernacularTitle:转化生长因子-?_1基因修饰树突状细胞诱导异种胰岛移植耐受
- Author:
Gang ZHAO
;
Fang WANG
;
Chunyou WANG
- Publication Type:Journal Article
- Keywords:
Islets of langerhans transplantation;
Diabetes mellitus;
Immune tolerance;
Transforming growth factor beta;
Dendritic cell
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether TGF-?_1 gene modified dendritic cells could induce immune tolerance in islet xenotransplantation. Methods Mature and immature DC were cultured from bone marrow of BALB/C mice, and then transfected the immature DC with TGF?_1 gene. Two kinds of DC were loaded with antigen of islet from Wistar rats, and injected into BALB/C mice with diabetes. After one week, the islets of Wistar or SD rats were transplanted under the capsular of left kidney. Survival time of grafts, proliferation and cytotoxicity of host T cell were evaluated. Results Grafts survival time in normal group was (10.2?1.1)d, (7.0?1.3)d in mature DC pretreated group (P0.05. The proliferation and cytotoxicity of T cell were enhanced in mature DC pretreated group (SI=6.92, KRmax=73%, P
