The effect of tissue factor expression on the invasive ability of human colon carcinoma cells
- VernacularTitle:组织因子表达对人类大肠癌细胞体外及体内侵袭能力的影响
- Author:
Hongwei YAO
;
Yuanlian WAN
;
Tao WU
;
Yisheng PAN
;
Xin WANG
;
Tonglin ZHANG
- Publication Type:Journal Article
- Keywords:
Colonic neoplasms;
Neoplasm invasiveness;
Tissue factor;
Transfection;
Microvessel density
- From:
Chinese Journal of General Surgery
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the role of tissue factor (TF) regulating the invasive ability of human colorectal carcinoma cells (HT-29). Method The plasmids pcDNA3.1/Zeo bearing either sense or antisense TFcDNA were transfected into HT-29 cells by lipofactamine 2000. TF proteins in transfectants and its parental cells were detected by Western blot. Matrigel invasion assays and nude mice inoculation assays were used to show the invasive ability of cancer cells in vitro and in vivo respectively. Microvessel density of the implanted tumors was counted. Result HT-29 cells with sense-TFcDNA transfection upregulated TF expression and invasive ability compared with the parental cells, and tumor tissues after sense transfection were of greater microvessel density than the parental cells. HT-29 cells transfected with antisense-TFcDNA had a downregulated TF expression and are of less microvessel density. Conclusion Positive TF expression increases the invasive ability of HT-29 cell in vitro and in vivo.
