Cytotoxicity of triamcinolone acetonide on human retinal pigment epithelial cells in vitro
- VernacularTitle:曲安奈德对正常人视网膜色素上皮细胞毒性的体外研究
- Author:
Ming ZOU
;
Junjun ZHANG
- Publication Type:Journal Article
- Keywords:
Pigment epithelium of eye;
Triamcinolone acetonide/pharmacology;
Proliferation;
Morphology
- From:
Chinese Journal of Ocular Fundus Diseases
2000;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cytotoxicity of triamcinolone acetonide (TA) on cultured human retinal pigment epithelial (RPE) cells. Methods Effect of TA with different concentraion (0.4, 0.2, 0.1, 0.05, and 0.025 mg/ml) on the proliferation of RPE cells was detected by methyl thiazolyl tetrazolial (MTT) assay; The changes of cellular cycle treated by TA with the drug concentration of IC(50) for 72 hours were measured by flow cytometry (FCM) ananlysis, and the morphological and ultrastructural changes of the cells were observed by phase-contrast microscopy and transmission electron microscopy. Results With the concentration of 0.4-0.025 mg/ml, TA inhibited the growth of RPE cells obviously in a dose-dependent manner compared with the control (P
