Effects of IL-1 ? on the Biological Activity of Human Periodontal Ligament Cells in Vitro
- VernacularTitle:白细胞介素-1?对人牙周膜细胞生物活性的影响
- Author:
Jianxin JI
;
Weijiao LIAO
;
Zhihui QIU
- Publication Type:Journal Article
- Keywords:
Periodontal ligament cells;
Interleukin 1?;
Fibronectin;
Alkaline phosphatase activity
- From:
Journal of Chinese Physician
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of interleukin- 1?(IL-1?)on the biological activity of human periodontal ligament(PDL) cells in vitro. Methods Human PDL cells were cultured in DMEM medium containing IL-1?(0.1,0.5,1,5 and 10ng/ml) for 1,2,3,4 and 5 days, respectively. The proliferation of PDL cells was measured by MTT assay at the first, second, third, fourth and fifth days after IL-1? treatment, respectively. Fibronectin level in the medium was determined by ELISA at the fourth days after 10ng/ml IL-1? treatment, and alkaline phosphatase(ALP) activity was measured by enzyme kinetic method. Results IL-1? inhibited the growth of human PDL cells in a dose-dependent manner, and its lowest effective concentration was 1ng/ml(P
