Determination of Isoniazid and Acetylisoniazid Concentration in Human Plasma by HPLC
- VernacularTitle:高效液相色谱法测定人血浆中异烟肼及乙酰异烟肼浓度
- Author:
Yiming XU
;
Jie WANG
;
Qiuyun ZHOU
;
Wenyu ZHU
- Publication Type:Journal Article
- Keywords:
Isoniazid;
Acetylisoniazid;
High performance liquid chromatography;
Precolumn derivatization
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for determination of the plasma concentrations of isoniazid(INH)and its metabolite acetyl-isoniazid(AcINH).METHODS:Trichloroacetic acid was added to plasma to precipitate protein,then the supernatant was divided into 2 parts,distilled water was added to the first part;while HCl was added to the second one,after incubated at 80℃for 1 hour,1%cinnamaldehyde was added to perform precolumn derivatization.The column was Hypersil BDS C 18 ,the mobil phase was eluted with0.02mol/L NaH 2 PO 4 (pH=4.0)-acetontrile(69∶31)and detected wave-length was 340nm,the flow fate was1ml/min.RESULTS:INH detectable concentration showed a good linear correlation in the range of 0.57～145.88?mol/L,the average recoveries of INH and AcINH were 101.1% and 101.5% respectively,the average RSD within or between day was less than 10%.CONCLUSION:The present method is applicable to monitoring of INH plasma concentration and the study of metabolism capacity of N-acetyltransferase.
