Identify Polysaccharide in Holothuria atra Jeager by Spectroscopy
- VernacularTitle:光谱法鉴定黑海参中的多糖成分
- Author:
Xican LI
;
Shaohui DU
;
Ling HUANG
;
Dongfeng CHEN
- Publication Type:Journal Article
- Keywords:
Holothuria atra Jeager;
Spectrosopy;
Identification;
Polysaccharide
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method to identify the polysaccharide element in Holothuria atra Jeager by spectroscopy.METHODS:The purity of polysaccharide in Holothuria atra Jeager was determined by electrophoretic method;the infrared absorption spectrum,ultraviolet absorption spectrum,carbon spectra and hydrogen spectra of NMR were identified in structure by infrared spectrophotometry,ultraviolet spectrophotometry and NMR spectrometry respectively.RESULTS:The gel band after electrophoresis took blue color and the results of spectrum determination accountable for each other.CONCLUSION:Uniformly constituent of polysaccharose was found in the Holothuria atra Jeager,which was an acidic polysaccharose with vitriolic esterfunction,without glucopeptide;?-configuration,?-glucopyranose was found and the intramolecular hydrogen bond was formed by hydroxide radical.
